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What is DNA preservation?
What is DNA?
How do I order PERPETUATE's DNA preservation services?
How long can DNA be preserved?
Who owns my DNA cell line after it has been preserved?
What happens if I want to discontinue preserving my pet's DNA?
How are tissue samples collected?
Will tissue sampling cause my pet pain?
For what species does PERPETUATE preserve DNA?
Is it possible to develop cell lines from any cellular material other than tissue?
What if my veterinarian refuses to take tissue samples?
When is the best time for collecting tissue?
Can viable tissue containing viable DNA be collected from a deceased animal?
What is a clone?
Why clone animals?
How is cloning accomplished?
How long will it be before my pet can be cloned?
Does a viable cell line guarantee a clone of my pet?
Why not delay tissue collection until pet cloning becomes more common?
What about human cloning?

DNA PRESERVATION

What is DNA preservation?

DNA preservation is the procedure that PERPETUATE utilizes to preserve viable DNA - that is, DNA that is able to grow - from pets. DNA preservation is also known as cell banking or gene banking.

What is DNA?

Deoxyribonucleic acid (DNA) is a nucleic acid that contains the genetic instructions for the biological development of a cellular form of life.

How do I order PERPETUATE's DNA preservation services?

Please contact PERPETUATE to order a DNA Collection Kit at phone number 808-989-2028 or order online right now.

How long can DNA be preserved?

Viable DNA can be preserved in liquid nitrogen for decades.

Who owns my DNA cell line after it has been preserved?

You maintain complete ownership of your pet's cell line after it has been developed and preserved.

What happens if I want to discontinue preserving my pet's DNA?

You may request PERPETUATE to continue dispose, preserve or transport your pet's cell line at any time.

TISSUE SAMPLING

How are tissue samples collected?

PERPETUATE ships a DNA Collection Kit to your veterinarian. The veterinarian uses kit materials to perform skin biopsies, then ships the tissue samples to our laboratory in Massachusetts by overnight courier.

Will tissue sampling cause my pet pain?

Tissue collection is a minor outpatient procedure that veterinarians usually do using a local anesthetic. There is no long-term pain and the healing process is relatively quick and pain-free. Some pet owners have skin biopsies taken when the pet is sedated for another procedure like teeth cleaning.

For what species does PERPETUATE preserve DNA?

Although the vast majority of our clients are dog and cat owners, we have developed cell lines on many different species of small animals and birds. PERPETUATE's business partner, Cyagra, Inc. preserves DNA from large animals such as horses and cattle.

Is it possible to develop cell lines from any cellular material other than tissue?

Cell lines are developed from blood cells. At least, 10 c.c.s of blood treated with heparin (to prevent coagulation) is required. Cell lines cannot be built from hair, teeth, bones and nails.

What if my veterinarian refuses to take tissue samples?

In our experience, most veterinarians respond positively to their clients' desires to PERPETUATE their pets. Your veterinarian should call us if he or she has any questions or reservations. If need be, we may be able recommend a veterinarian near you who has taken tissue samples for our clients.

When is the best time for collecting tissue?

Today! Take tissue samples when your pet is healthy and strong.

Can viable tissue containing viable DNA be collected from a deceased animal?

Remember, a viable DNA sample means one that has the ability to grow. Although we have been able to process tissue from deceased animals within six days of their death, this is not recommended. The sooner that the tissue samples are taken and reach the laboratory, the greater the chances of developing a viable cell line that will eventually result in your healthy future pet. See EMERGENCIES.

ANIMAL CLONING

What is a clone?

A clone is an identical twin that has the identical genetic makeup (DNA) of its genetic donor. Nature also exhibits selected cloning such as bacteria.

Why clone animals?

Cloning is the ultimate breeding technology. Pet owners routinely attempt to transfer desired traits from their exceptional pets through selective breeding. With luck, their new puppy or kitten will exhibit desired genetic traits and be of the preferred sex. Cloning removes the luck factor: clones are genetic duplicates and of the same sex as the donor pet!

How is cloning accomplished?

Cloning is accomplished through a process called nuclear transfer. Scientists remove the nucleus of a female oocyte (egg cell) and replace its nucleus with DNA from the genetic donor's cells. The new cell is activated in the laboratory, begins to divide, and forms an embryo. The embryo is then implanted into a surrogate mother dog or cat for normal gestation and delivery.

How long will it be before my pet can be cloned?

It is unclear whether pet cloning will become commercially viable. In 1996, a sheep named Dolly was cloned in Scotland. Since then, scientists have cloned goats, horses, cattle, mice, pigs, cats and dogs. Cloning dogs and cats is tough compared to cloning livestock.

Does a viable cell line guarantee a clone of my pet?

A viable cell line will not necessarily guarantee you a clone of your pet. Pet cloning must first become technically and commercially more feasible. Second, cattle-cloning scientists have not been able to produce cattle clones from all viable cell lines for known and unknown reasons. The fact is, however, that pet clones cannot be produced without first establishing a viable cell line.

Why not delay tissue collection until pet cloning becomes more common?

Many animal owners cannot or are not willing to risk the loss of their pet before cloning becomes a reality. Furthermore, some experts believe that cells taken from young animals may be more responsive to cloning.

What about human cloning?

PERPETUATE 's services are absolutely restricted to animals. We are not involved in human cloning in any way.

To enter your special pet in our program, call 808-989-2028

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